"No locks available" error

[imbertj]

Hello, I am running MiXCR with the exome-seq preset on 83 different bam files simultaneously and have been getting this error message at random. It happens for some cells but not others, and if I rerun MiXCR on the cells that failed, the second or third run usually works and I don't get the error message. The error message still comes up if I run MiXCR on just 15 bam files simultaneously.

java.lang.RuntimeException: java.io.IOException: No locks available
at com.milaboratory.o.cG.a(SourceFile:137)
at com.milaboratory.o.cJ$a.run(SourceFile:484)
Caused by: java.io.IOException: No locks available
at java.base/sun.nio.ch.FileDispatcherImpl.lock0(Native Method)
at java.base/sun.nio.ch.FileDispatcherImpl.lock(FileDispatcherImpl.java:96)
at java.base/sun.nio.ch.FileChannelImpl.lock(FileChannelImpl.java:1119)
at java.base/java.nio.channels.FileChannel.lock(FileChannel.java:1063)
at com.milaboratory.o.cG.a(SourceFile:116)
... 1 more
License manager thread died.
License error: UnknownError

Does anyone know how to fix this?

Thank you!

[mizraelson]

Hi, what version of MiXCR did you use?

[imbertj]

Hi, thank you for your very quick reply! I'm using

MiXCR v4.7.0
RepSeq.IO v2.5.0
MiLib v3.5.0
Built-in V/D/J/C library: repseqio.v5.1

I'm using the following Java version:

openjdk 11.0.26 2025-01-21
OpenJDK Runtime Environment (build 11.0.26+4-post-Ubuntu-1ubuntu122.04)
OpenJDK 64-Bit Server VM (build 11.0.26+4-post-Ubuntu-1ubuntu122.04, mixed mode, sharing)

I only got my licence in January 2025, so it shouldn't be expired, but do you think it's worth renewing it in case it's corrupted?

Thank you for your help!

[imbertj]

Hi, I am still encountering this error message. Please could you let me know if you have any suggestions for fixing it? Thank you!